In order to consistently provide our customers with access to the highest quality antibody reagents, MBS has developed and refined our cGMP protein A purification and downstream processing protocols. Our protocols offer the optimum binding and gentlest elution conditions based on immunoglobulin subclass. Avoiding the harsh, low-pH elution conditions typically used can prevent irreversible IgG aggregate formation. Our protein A purification process is typically able to achieve monomer IgG purity of 95% or greater.
Protein A Purification Highlights:
Suitable for murine monoclonal antibody productions in ascites and roller bottles
Optimized binding and elution conditions based on mouse IgG subclass
Typical monomer IgG purity of 95% or greater
Minimal irreversible aggregate formation to confound assays
In-house purification process accommodates small to large scale productions
- Custom buffer and concentration requirements can be requested