Low Endotoxin Purification

Endotoxin is a complex aggregate of acidic lipopolysaccharides (LPS) and is continuously shed from the outer membrane of viable gram-negative bacteria. Although bacteria are often removed by using a 0.2 μm sterilizing grade filter, LPS itself is difficult to remove or inactivate because it is extremely heat and pH stable. When producing antibodies for in vivo studies and cell-based assays, it is often necessary to minimize endotoxin concentration to prevent false readings. Removal of endotoxin is one of the most difficult downstream processes during antibody purification. At MBS we have developed a low endotoxin purification process that serves to minimize bacterial exposure, thus limiting endotoxin loads in the final purified product. Our approach preserves maximal antibody yield and function, something that is often sacrificed with many of the commercially available endotoxin removal kits on the market.

Low Endotoxin Purification Highlights:

  • Process developed to minimize bacterial and endotoxin exposure

  • ŸMBS approach maximizes antibody yield and function

  • FinalŸ endotoxin concentration reported (EU/mg)