Antibody Characterization Services

Determination of an antibody’s unique characteristics is essential for the development of a successful assay. Customers may also consider antibody characterization services to confirm lot to lot consistency. Antibody characterization services available at MBS include:

Enzyme-Linked Immunosorbent Assay (ELISA)

Confirm antibody specificity to the customer supplied antigen in an immunoassay format.

Western Blot

Confirm antibody specificity to the customer supplied antigen in an immunoblot format.

Size Exclusion Chromatography (SEC)

MBS offers purity analysis of antibody samples by size exclusion chromatography. SEC separates monomeric IgG from dimeric, aggregated, and fragmented antibody under native conditions. A percent purity is calculated by peak integration and a report is provided.

Learn More about MBS SEC Polishing when the highest level of purity is necessary


SDS-PAGE evaluates purified antibody samples under non-reducing and reducing denatured conditions. This electrophoretic characterization identifies intact IgG as well as heavy and light chain components.

Isoelectric Focusing (IEF) 

IEF analysis is run on purified monoclonal antibody samples to determine the isoelectric point (pI). This is the pH at which the antibody carries no net electrical charge and can be useful in determining suitable buffer conditions.  The electrophoresis technique can resolve an isoform profile which can be used for evaluating lot to lot consistency.

Endotoxin Testing 

MBS offers quantitative LAL endotoxin testing of samples to determine the EU/mg bioburden. This service is offered using the Endosafe®-PTS system.

Contact MBS today to learn more about antibody characterization services: 207-797-5454 or