Quantity: 250ug  $210.00   1mg $300.00

MAB222P Monoclonal Antibody to Human Osteopontin 

Background    Human Osteopontin (OPN) is a negatively charged, hydrophilic protein of 314 amino acids and is subject to significant post translational modifications.  OPN is cleaved by members of the matrix metalloproteinase family (MMP) which results in the generation of N-terminal and C-terminal OPN fragments.  The newly exposed SVVYG epitope on the N-terminal fragment has been shown to participate in cell adhesion1.

Clone Determination  14C08

Host  OPN Knockout Mouse

Immunogen   DSVVYG peptide conjugated to KLH          

Immunoglobulin Class   IgG1 κ

Specificity   Recognizes native human OPN from purified breast milk.  Specific for the MMP cleavage site, DSVVYG, of human OPN. 

Purification   Protein A chromatography from in vitro production

pI  6.5-7.5

Buffer   0.15M PBS                                                                                                             

Applications   ELISA- sandwich and indirect, Pairs well with MAB193P

Storage   Store at -20C to -80C; avoid repeat freeze- thaw cycles 


1Expression and characterization of recombinant osteopontin peptides representing matrix metalloproteinase proteolytic fragments

(2004) Matrix Biology, 23 (7), pp. 457-466.

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