MAB194P Monoclonal Antibody to Human Osteopontin, N-terminal
Background Human Osteopontin (OPN) is a negatively charged, hydrophilic protein of 314 amino acids and is subject to significant post translational modifications (PTMs) including phosphorylation and glycosylation. Due to its acidic nature and PTMs, OPN runs anomalously by SDS-PAGE. Although its mass is 35kD, apparent molecular weights may range up to 75kD. In addition, OPN is subject to proteolytic modification into smaller molecular weight fragments.
Clone Determination 2F10
Immunogen Recombinant full-length human Osteopontin
Immunoglobulin Class IgG1 κ
Specificity Specific for the N-terminal fragment of full-length Osteopontin. Recognizes native human Osteopontin from purified breast milk and recombinant murine OPN expressed in E.coli.
Purification Protein A chromatography from ascites
Buffer 0.15M PBS
Applications ELISA, WB
Storage Store at -20C to -80C; avoid repeat freeze- thaw cycles
Alicia Plumer, Hongyi Duan, Sripriya Subramaniam, F. Lee Lucas, Susan Meisfeldt, Ah-Kau Ng, Lucy Liaw. Development of Fragment Specific Osteopontin Antibodies and ELISA for Quantification in Human Metastatic Breast Cancer. BMC Cancer 2008, 8:38.
FOR RESEARCH AND IN VITRO USE ONLY