Ascites Antibody Production

MBS offers in vivo antibody production of monoclonal antibodies in our cGMP facility.  Antibody productions through mouse ascites provide customers with a cost-effective option for large scale antibody productions. Production schedules are customizable and projects can typically be started within two weeks of booking. MBS ascites production technicians follow carefully regulated operating procedures for the efficient delivery of antibody for any application.

In Vivo Monoclonal Antibody Production via Mouse Ascites (6-8 Weeks)

  • All incoming cell lines require mycoplasma testing. Mycoplasma decontamination services are available for contaminated cell lines.

  • BALB/c, BALB/c hybrid, Nude and SCID mice are available; additional strains available upon request

  • Average yield from BALB/c mice is 3-5mLs of ascites per mouse with an average antibody concentration of
    3-5mg/mL

  • Ascites assayed for total protein content and estimated IgG concentration

MBS also offers in vitro production methods when smaller quantities of antibody are needed or a preparation free of endogenous mouse antibodies is required and the option to produce in serum free media is preferred.

Learn more about in vitro production options here.

Learn more about MBS antibody purification services here